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RGUHS Nat. J. Pub. Heal. Sci Vol No: 11 Issue No: 1  pISSN: 2249-2194

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Original Article

COMPARITIVE CHROMATOGRAPHIC ANALYSIS OF HYDROALCOHOLIC AND SUPERCRITICAL FLUID EXTRACTS OF USHEERA (VETIVERIA ZIZANIOIDES LINN).

Ashalatha M1, Lalitha B.R2, Subhashini N S*,3,

1Ashalatha M, Retired Principal, Department of Dravyaguna, Government Ayurveda Medical College, Bengaluru.

2Lalitha B.R.,Professor and HOD, Department of Dravyaguna, Government Ayurveda Medical College, Bengaluru, Karnataka, India.

3Subhashini N S., Discovery Science group, Research and Development Centre, The Himalaya Drug Company, Bengaluru. Email- subhashiniputtur@gmail.com

*Corresponding Author:

Subhashini N S., Discovery Science group, Research and Development Centre, The Himalaya Drug Company, Bengaluru. Email- subhashiniputtur@gmail.com, Email: subhashiniputtur@gmail.com
Received Date: 2017-05-10,
Accepted Date: 2017-06-30,
Published Date: 2017-07-31
Year: 2017, Volume: 4, Issue: 2, Page no. 12-15,
Views: 362, Downloads: 8
Licensing Information:
CC BY NC 4.0 ICON
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0.
Abstract

TLC and HPTLC analysis were carried out for the hydro alcoholic and supercritical fluid extacts of fresh and dried sample of Usheera.

In TLC, all the four extracts revealed a green coloured band ar Rf 0.84 corresponding to the marker compound khusenol. In HPTCL, at 254nm supercrtitical extracts (S.C.F.E.) had more number of peaks when compared to hydroaclcoholic extracts(H.A.) and at 366nm hydroalcoholic extracts had more number of peaks indicating that more constituents were soluble in S.C.F.E.

 

<p>TLC and HPTLC analysis were carried out for the hydro alcoholic and supercritical fluid extacts of fresh and dried sample of Usheera.</p> <p>In TLC, all the four extracts revealed a <strong>green coloured </strong>band ar<strong> Rf 0.84</strong> corresponding to the marker compound <strong>khusenol.</strong> In HPTCL, at 254nm supercrtitical extracts (S.C.F.E.) had more number of peaks when compared to hydroaclcoholic extracts(H.A.) and at 366nm hydroalcoholic extracts had more number of peaks indicating that more constituents were soluble in S.C.F.E.</p> <p>&nbsp;</p>
Keywords
Usheera (Vetiveria zizanioides Linn.), TLC and HPTLC.
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